Ready-to-use and no yolk and antibiotics. Containing 28% (v/v) glycerol and 2% HAS

Base solution HEPES/MOPS double buffering

Semen: volume ratio of refrigerating solution: 3:1

Reducing the dilution of low count sperm samples

Adding antioxidants to protect sperm

Adding the key amino acids-glycine, adenylate and glutamine